Scientist I: CLD Small Scale Bioreactor SME
An exciting opportunity is available for a Cell Line Development Scientist to join a growing start-up. With state-of-the-art facilities and a robust pipeline of upcoming projects, this role offers the chance to contribute to innovative research and development.
Responsibilities:
Develop and execute small scale (Ambr15 & 24/96 deep well) fed-batch methods to screen and validate cell lines as part of an innovative multidisciplinary team. Author SOPs, training documents & COSHH forms as key deliverables. Train team members to a high degree of competence to ensure data integrity. Document research findings clearly in a lab book. Communicate regularly in group meetings and prepare and deliver presentations to project team and internal stakeholders. Provide routine laboratory maintenance, including participating in equipment upkeep, cleaning rotas and laboratory consumables stock management. Shared responsibility for out-of-hours cell culture maintenance. Ensure that laboratory health, safety and quality procedures are followed. Support the preparation of laboratory risk assessments and maintenance of health and safety protocols. Maintain the confidentiality of the project and protocols. Keep abreast of current technologies and relevant scientific literature to help identify opportunities for improvements and reduction of costs. Minimum Required Qualifications
BSc/MSc in Biochemistry, Molecular Biology or related field. BSc/MSc with 5+ years of industry experience or PhD with 2+ years’ experience. Essential Experience:
Extensive experience of working with small scale bioreactor systems including Ambr15. Experience of developing small scale mammalian cell culture fed-batch models systems using deep well microplates. Experience of screening clonal cell lines for optimal performance in batch, fed-batch and perfusion bioreactor systems. Experience of optimizing fed-batch methods for antibody bioproduction. Experience of working with bioprocess relevant cell lines including CHO and HEK293. Experience of performing analytical methods to characterize cell lines for high antibody titre, qP and CQA such as HPLC/UPLC & LC-MS. Application of Design of Experiments (DOE) to rationalize experiment design. Skilled in experimental design and troubleshooting. Experience of writing SOPs, training documents and risk assessments. Experience of training other team members to a high level of competency. Familiarity with mammalian cell line development including generation of GM/GE cell lines, single cell cloning and CRISPR / ORF library screens. Evidence of executing a range of molecular and cell biology assays to a high standard including FACS, qRT-PCR, Western Blotting & ELISA. Ability to work independently and as part of a team. Experience of planning experiments within a given timeframe. Good numeracy, computer and communication skills. Desirable Experience:
Experience with automation and high-throughput technologies applied to mammalian cell line generation eg. Beacon, 96 deep well plate model systems & Ambr15. Familiarity with working with large datasets including transcriptomic / proteomic datasets. Experience of mammalian cell culture and related techniques including passaging, banking, transfection, nucleofection & genotyping. Experience of working safely with 3rd generation lentiviral plasmid systems within a CAT2.0 environment. Skills and Personality:
Innovative mindset. Advanced problem-solving skills. Enthusiasm and initiative. Driven to deliver. Reliable and responsible. Able to multitask. Good time management and organization skills. Excellent communication skills both written and spoken. Happy in a growing and multi-disciplinary team. Job Info
Job Title:
Cell Line Development Scientist Company:
CV-Library Location:
Oxford, Oxfordshire Posted: Closes:
Jan 19th 2025 Sector:
Medical, Pharmaceutical & Scientific Contract:
Permanent Hours:
Full Time
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Develop and execute small scale (Ambr15 & 24/96 deep well) fed-batch methods to screen and validate cell lines as part of an innovative multidisciplinary team. Author SOPs, training documents & COSHH forms as key deliverables. Train team members to a high degree of competence to ensure data integrity. Document research findings clearly in a lab book. Communicate regularly in group meetings and prepare and deliver presentations to project team and internal stakeholders. Provide routine laboratory maintenance, including participating in equipment upkeep, cleaning rotas and laboratory consumables stock management. Shared responsibility for out-of-hours cell culture maintenance. Ensure that laboratory health, safety and quality procedures are followed. Support the preparation of laboratory risk assessments and maintenance of health and safety protocols. Maintain the confidentiality of the project and protocols. Keep abreast of current technologies and relevant scientific literature to help identify opportunities for improvements and reduction of costs. Minimum Required Qualifications
BSc/MSc in Biochemistry, Molecular Biology or related field. BSc/MSc with 5+ years of industry experience or PhD with 2+ years’ experience. Essential Experience:
Extensive experience of working with small scale bioreactor systems including Ambr15. Experience of developing small scale mammalian cell culture fed-batch models systems using deep well microplates. Experience of screening clonal cell lines for optimal performance in batch, fed-batch and perfusion bioreactor systems. Experience of optimizing fed-batch methods for antibody bioproduction. Experience of working with bioprocess relevant cell lines including CHO and HEK293. Experience of performing analytical methods to characterize cell lines for high antibody titre, qP and CQA such as HPLC/UPLC & LC-MS. Application of Design of Experiments (DOE) to rationalize experiment design. Skilled in experimental design and troubleshooting. Experience of writing SOPs, training documents and risk assessments. Experience of training other team members to a high level of competency. Familiarity with mammalian cell line development including generation of GM/GE cell lines, single cell cloning and CRISPR / ORF library screens. Evidence of executing a range of molecular and cell biology assays to a high standard including FACS, qRT-PCR, Western Blotting & ELISA. Ability to work independently and as part of a team. Experience of planning experiments within a given timeframe. Good numeracy, computer and communication skills. Desirable Experience:
Experience with automation and high-throughput technologies applied to mammalian cell line generation eg. Beacon, 96 deep well plate model systems & Ambr15. Familiarity with working with large datasets including transcriptomic / proteomic datasets. Experience of mammalian cell culture and related techniques including passaging, banking, transfection, nucleofection & genotyping. Experience of working safely with 3rd generation lentiviral plasmid systems within a CAT2.0 environment. Skills and Personality:
Innovative mindset. Advanced problem-solving skills. Enthusiasm and initiative. Driven to deliver. Reliable and responsible. Able to multitask. Good time management and organization skills. Excellent communication skills both written and spoken. Happy in a growing and multi-disciplinary team. Job Info
Job Title:
Cell Line Development Scientist Company:
CV-Library Location:
Oxford, Oxfordshire Posted: Closes:
Jan 19th 2025 Sector:
Medical, Pharmaceutical & Scientific Contract:
Permanent Hours:
Full Time
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