About the Role
We are seeking a highly skilled and motivated Senior Laboratory Research Scientist to join our Proteomics Scientific Technology Platform (STP) at the Francis Crick Institute. This is a lab-based position responsible for driving forward qualitative and quantitative proteomic and clinical proteomic projects, from experimental design through sample preparation to data analysis. Key Responsibilities
Planning of experiments, decision-making about which pipeline to take, and independently executing experiments for projects. Performing both standard and bespoke proteomics experiments starting with sample preparation from a variety of biological sources using common biochemical methodologies for cell lysis, SDS-PAGE, in-gel, in-solution, TCA and acetone precipitation digest protocols, TMT labeling, high pH chromatographic fractionation and label-free quantification. LC-MS/MS nanoUHPLC data acquisition, evaluation, and processing of raw data. Requirements
The post holder should embody and demonstrate our core Crick values:
bold, open, collegial , in addition to the following: A PhD or equivalent experience gained through working in a mass spectrometry laboratory, including basic and advanced proteomic sample handling and processing protocols, and familiarity with instrument maintenance requirements. Detailed understanding of proteomics-related bioinformatics and associated software packages such as PEAKS, Mascot, Scaffold, MaxQuant, Spectronaut, DIA-NN, and Skyline. Experience with developing, optimizing, and applying next-generation proteomics and mass spectrometry methods such as DIA, DDA, label-free profiling, TMT, PRM, etc. Salary and Benefits
The salary for this role is estimated to be around £60,000 - £80,000 per annum, depending on experience. In addition to a competitive salary, we offer a comprehensive benefits package, including pension scheme, annual leave, and training opportunities.
We are seeking a highly skilled and motivated Senior Laboratory Research Scientist to join our Proteomics Scientific Technology Platform (STP) at the Francis Crick Institute. This is a lab-based position responsible for driving forward qualitative and quantitative proteomic and clinical proteomic projects, from experimental design through sample preparation to data analysis. Key Responsibilities
Planning of experiments, decision-making about which pipeline to take, and independently executing experiments for projects. Performing both standard and bespoke proteomics experiments starting with sample preparation from a variety of biological sources using common biochemical methodologies for cell lysis, SDS-PAGE, in-gel, in-solution, TCA and acetone precipitation digest protocols, TMT labeling, high pH chromatographic fractionation and label-free quantification. LC-MS/MS nanoUHPLC data acquisition, evaluation, and processing of raw data. Requirements
The post holder should embody and demonstrate our core Crick values:
bold, open, collegial , in addition to the following: A PhD or equivalent experience gained through working in a mass spectrometry laboratory, including basic and advanced proteomic sample handling and processing protocols, and familiarity with instrument maintenance requirements. Detailed understanding of proteomics-related bioinformatics and associated software packages such as PEAKS, Mascot, Scaffold, MaxQuant, Spectronaut, DIA-NN, and Skyline. Experience with developing, optimizing, and applying next-generation proteomics and mass spectrometry methods such as DIA, DDA, label-free profiling, TMT, PRM, etc. Salary and Benefits
The salary for this role is estimated to be around £60,000 - £80,000 per annum, depending on experience. In addition to a competitive salary, we offer a comprehensive benefits package, including pension scheme, annual leave, and training opportunities.
